Histotech - Electron Microscopy Specialist

Melissa Owens| President 

P: (386)339-0839 | E: [email protected]

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Job ID: 3583147

Job Title: Histotech – Electron Microscopy Specialist

Department:  Anatomic Pathology / Diagnostic Electron Microscopy

Location:  Miami, FL

Schedule:  Full-Time, Clinical

Position Summary

The EM Specialist is a subject matter expert responsible for the end-to-end processing and high-resolution imaging of clinical specimens (e.g., renal biopsies, cilia, and neuromuscular tissues). This role requires advanced technical mastery of ultramicrotomy and Transmission Electron Microscopy (TEM) to provide critical diagnostic data for Pathologist review. 

Requirements:

• Experience: Minimum of 3–5 years of hands-on clinical Transmission Electron Microscopy experience in a pathology or diagnostic laboratory
• Education: Associate’s or Bachelor’s degree in Histotechnology, Biological Sciences, or a related field
• Certification: HTL(ASCP) preferred and Certified Electron Microscopy Technician (CEMT) through the Microscopy Society of America (MSA) highly desirable
• Technical Skills:

Expert-level ultramicrotomy

Diamond knife handling and grid manipulation

TEM operation and image acquisition

• Regulatory Knowledge: CAP and CLIA compliance standards
• LIS: Laboratory Information System experience not required; training provided

Job Description

Key Responsibilities

• Expert Specimen Preparation: Perform advanced clinical EM preparation, including precise chemical fixation, osmium tetroxide post-fixation, dehydration, and epoxy resin embedding
• Precision Ultramicrotomy: Produce semi-thin and ultra-thin sections using diamond knives; mount grids with extreme precision while preventing artifacts
• Diagnostic Imaging: Operate high-voltage Transmission Electron Microscopes to identify and document diagnostic ultrastructural findings such as glomerular basement membrane changes, ciliary abnormalities, neuromuscular junction defects, and viral particles
• Specialized Staining & Techniques:

Perform heavy-metal contrasting with uranyl acetate and lead citrate; conduct specialized techniques including immunogold labeling and negative staining as required

Quality Assurance & Compliance**

Adhere to CAP and CLIA standards, maintain detailed documentation, and manage hazardous chemical handling and disposal (e.g., osmium tetroxide, cacodylate buffers)

• Equipment Maintenance: Conduct advanced calibration, troubleshooting, and preventive maintenance of electron microscopes, ultramicrotomes, and related EM equipment

Physical & Working Conditions

• Handling of acutely toxic chemicals and sharp objects (diamond knives, glass breakers).
• Ability to sit for extended periods in a darkroom environment for microscope operation.